Reactive oxygen species (ROS) damage proteins, lipid membranes, and nucleic acids (DNA and RNA), all critical functional components of living cells. The progressive accumulation of unrepaired oxidative (free radical) damage over time is believed to be a major contributor to the aging process and to a variety of age-related chronic diseases. Oxidative DNA damage can result from numerous endogenous metabolic processes as well as from exposure to environmental and dietary oxidants. DNA damage may manifest itself as deviations from the normal, intact structure which may, if left unrepaired, result in a mutation or block DNA replication. These deviations may be caused by physical or chemical agents and occur naturally or artificially.
In humans, oxidative DNA damage to has been estimated at 104 hits per cell per day. Quantitative estimation of modified bases in urine is a useful means of assessing the amount of DNA damage. KS has developed urine-based DNA damage assays for 5-Hydroxymethyl-2'-deoxyuridine (HMdU), 8-hydroxyguanosine (8-OHG) and 8-hydroxy-2-deoxy Guanosine (8-OH-dG) by LC/MS/MS methodology. Available as a panel or individually, these DNA damage assays have been used in conjunction with studies of aging, cardiovascular disease, neurodegenerative disease and cancer.
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8-hydroxy Guanosine
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8-Hydroxy-2-deoxy guanosine
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DNA Damage Assays by LC/MS/MS
Matrix: Urine (1 mL urine cup) SHIP FROZEN
8-hydroxy Guanosine
CAS 3868-31-3
Synonyms: 8-OHG, 8-OH-guanosine, 8-Oxoguanosine, 7,8-dihydro-8-oxo-Guanosine, 8-Oxoguanosine, 8-hydroxyguanosine, Purine-6,8(1H,9H)-dione
8-hydroxyguanosine (8-OHG) is a marker for measuring the rate of oxidative damage to nucleic acids and lipids. Recently, 8-hydroxyguanosine has generated considerable interest as a product of hydroxyl radical attack on RNA that can be used to estimate RNA damage in humans. Products such as 8-hydroxy guanosine are used for these estimates. 8-hydroxy guanosine (8-OHG) has been found to be associated with neurodegenerative disorders. 8-hydroxy guanosine was found in the cerebral cortex in three of six cases of neuropathologically examined autopsy of subacute sclerosing panencephalitis patients. This condition is caused by persistent brain infection of mutated measles virus, showing inflammation, neuronal loss, and demyelination. The concentration of 8-OHG in CSF in Parkinson's disease (PD) patients is approximately three-fold that in controls.
8-Hydroxy-2-deoxy guanosine
CAS 88847-89-6
Synonyms: 8-OH-dG, 8-OHdG, 8-Oxo-7,8-dihydro-2'-deoxyguanosine, 8-Oxo-7,8-dihydrodeoxyguanosine, 8-Oxo-dG, 8-hydroxydeoxyguanosine, oh8dG
8-hydroxy-2-deoxy guanosine (8-OH-dG) is a product of oxidative DNA damage by reactive oxygen species and serves as an established marker of oxidative stress. Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors. Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension.